BioDefense
Food Security Testing
Water Security Testing
Mutation Discovery
  - LightScanner
  - HR-1
  - RapidCycler 2
  - LightScanner Master Mixes
  - LCGreen Melting Dyes
  - LightScanner Assays
  - Custom Probes & Primers
  - Reagents, Buffers & Capillary Tubes
Genotyping
Mutation Discovery

Mutations in PCR products are detected by the presence of heteroduplexes optimally detected by our LCGreen® Plus dye. Variations are identified by changes in the shape of the melting profile compared to a reference sample. Hi-Res Melting™ on the LightScanner is also being used for mutation discovery in tumor samples, yielding information on mutations present in low allele fractions (as low as 5%) and allelic imbalances between tumor and matched normal samples.

Use the links below to learn more about our complete line of Mutation Discovery products.

 

Instrument

LightScanner Instrument
LightScanner® System

  • Hi-Res Melting Instrument
  • 96- or 384-well plate system
  • Results in less than five minutes
  • Software automatically analyses each sample and defines the standards and variants

HR-1 Instrument
HR-1® System

  • Hi-Res Melting Instrument
  • Single sample capillary melter
  • Up to 45 samples per hour with a 0.3°C ramp rate
  • Easy-to-use, low cost mutation discovery instrument

RapidCycler 2 Instrument
RapidCycler® 2 Instrument

  • High-Speed PCR
  • 30 cycles in under 15 minutes
  • 5-20 µl using 1.5 mm capillary tubes
  • 48 samples per run, over 1,500 samples per 8 hour day
Chemistry

LightScanner Master Mix
LightScanner Master Mix

  • A pre-optimized solution
    specifically designed to deliver superior PCR performance for Hi-Res Melting applications on the LightScanner Instrument

High Sensitivity Master Mix
High Sensitivity Master Mix

  • The LightScanner® High Sensitivity Genotyping Master Mix is a specialized master mix containing LCGreen Plus® dye and internal temperature calibrators.

Warfarin Genotyping Reagents
Warfarin Genotyping Reagents

  • Our Warfarin Genotyping Reagents (ASR) provide a fast and effective solution for genotyping.

LCGreen + Melting Dye
LCGreen® Melting Dyes

  • LCGreen dyes are specifically designed for high-resolution melting curve analysis to detect DNA sequence variants (mutations, polymorphisms, etc).

LCGreen + Melting Dye
Reagents, Buffers and Capillary Tubes

 

 

 

Services

LightScanner Assay Protocols
LightScanner Assays

  • Free assay design protocols that have been optimized for Hi-Res Melting.

IT BioChem - Custom Probes, Primers and Melting Dyes
Custom Probes & Primers

  • Order custom designed probes, primers and melting dyes for DNA amplificaiton, mutation and detection.
  • ASR grade oligos
  • Manufactured under cGMP