This master mix incorporates a Taq polymerase and an antibody-based hot-start method, dNTPs, magnesium chloride and Idaho Technology's proprietary LCGreen® PLUS dye and is formulated at a 2.5X concentration. The antibody-mediated hot-start method means no enzyme pre-activation is required prior to or during thermocycling, translating into much faster PCR protocol times than other methods of hot-start protection.

The master mix includes a concentration of magnesium that is optimized to work for almost all reactions. Additional magnesium chloride solution is provided for further optimization if required. The master mix is also compatible with most additives used to enhance amplification efficiency of high GC amplicons.

This High Sensitivity Master Mix is a pre-optimized master mix designed for Hi-Res Melting® and subsequent genotyping of small amplicons. This kit is specifically designed for mutation genotyping on the LightScanner instrument.

This master mix incorporates specially tested Taq polymerase and an antibody-based hot-start method, dNTPs, magnesium chloride, and Idaho Technology’s proprietary LCGreen® Plus dye. It is formulated at a 2.5X concentration. The antibody hot-start method means that no enzyme pre-activation is required before thermocycling. In addition, it includes special nucleic acid-based internal calibrators at optimal concentrations to provide adequate signal when used with
amplicons up to 120 bp in size.

The master mix includes a universal magnesium buffer designed to work for almost all reactions. Additional magnesium chloride solution is provided for further optimization, if required. The master mix is also compatible with most additives used to enhance amplification efficiency of high G-C amplicons, although some additives may diminish hot-start efficacy. In general, only use additives, like DMSO or betaine, when necessary.

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This Genotyping Master Mix is a pre-optimized master mix specially designed for genotyping experiments not requiring an enzyme with exonuclease activity. This master mix is compatible with technologies like SimpleProbes®, where the genotype is determined via melting temperature of the SimpleProbe. This product is intended for laboratory use only.

This master mix includes Taq polymerase, magnesium chloride, and bovine serum albumin (BSA) and is formulated at a 2.5X concentration. Enzyme preactivation (i.e., high temperature, hot start, incubation) is not required before thermocycling.

This master mix has been tested on all Idaho Technology real-time PCR instruments, Roche LightCycler®, and many commercially available heat block thermal cyclers.