BioSurveillance
Food Security Testing
Water Security Testing
Mutation Discovery
Genotyping
  - LightScanner
  - LightScanner 32
  - LightScanner Master Mixes
  - LCGreen Plus Melting Dye
  - LightScanner Assays
  - Reagents, Buffers & Capillary Tubes
FilmArray
LightScanner® System

Mutation Discovery, Gene Scanning and Genotyping

The LightScanner is the first system to use Hi-Res Melting® (also known as High Resolution Melting, or HRM) analysis for mutation scanning and genotyping in 96- or 384-well plate system. Standardization is important for research, making the plate format preferable in many settings. It is compatible with nearly all thermal cyclers and qPCR machines.

The LightScanner is unsurpassed in its temperature control for plate-based systems. It also has the highest data quality. With superb sensitivity and specificity, this instrument is designed to meet the needs of high-throughput scanning projects. Simply add LCGreen® Plus dye to the initial PCR reaction– then transfer the PCR plate to the LightScanner for melting. Intuitive analysis software groups the results and provides automatic results.

Benefits from a Complete Product Solution

Instrument   Chemistry
  Results in less than five minutes   Optimized Master Mix for superior PCR performance for Hi-Res Melting applications
  Hi-Res Melting is non-destructive allowing samples to be recovered for further analysis
Support Software
  Obtain quick assistance through phone and online help desks   Software automatically analyses each sample and defines the standards and variants
  Work directly with highly experienced scientists   Simple data management tools
  Primer design software
 

LightScanner Software

Our comprehensive software package provides both assay design and data analysis modules. The primer design module streamlines assay design for Hi-Res Melting. The analysis software features Call-IT®, a patented scientific method where the software critically analyses each sample, defines and groups wild-types and variants. This software also provides users with simple data management tools.

 

 

High Resolution Melting References

The LightScanner Hi-Res Melting system has been used for over five years and has resulted in approximately 200 peer-reviewed publications, making it the most highly published Hi-Res Melting instrument. Here are a few select references for your review.

  1. De Koeyer D, Douglass K, Murphy A, Whitney S, Nolan L, Song Y, De Jong W (2010). Application of high-resolution DNA melting for genotyping and variant scanning of diploid and autotetraploid potato. Mol Breeding. 25, 67–90.

  2. Rolf H.A.M. Vossen, Emmelien Aten, Anja Roos, Johan T. den Dunnen. (2009). High-Resolution Melting Analysis (HRMA) - More than just sequence variant screening Human Mut. 30, 860-866.

  3. Parant JM, George SA, Pryor R, Wittwer CT, Yost HJ. (2009). A rapid and efficient method of genotyping zebrafish mutants. Dev Dyn. 238,3168-74.

  4. van der Stoep N, van Paridon CDM, Janssens T, Krenkova P, Stambergova A, Macek M, Matthijs G, Bakker E. (2009). Diagnostic guidelines for high-resolution melting curve (HRM) analysis: An interlaboratory validation of BRCA1 mutation scanning using the 96-well LightScanner. Human Mut. 30, 899-909.

  5. Aten E, White SJ, Kalf ME, Vossen RH, Thygesen HH, Ruivenkamp CA, Kriek M, Breuning MH, den Dunnen JT.(2008). Methods to detect CNVs in the human genome. Cytogenet Genome Res. 123, 313-21.

  6. Audrezet MP, Dabricot A, Le Marechal C, Ferec C. (2008). Validation of high-resolution DNA melting analysis for mutation scanning of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. J Mol Diagn. 10, 424-34.

  7. C. Voegele, S.V. Tavtigian, D. de Silva, Cuber Thomas, F. Le Calvez-Kelm. (2007). A Laboratory Information Management System (LIMS) for a high throughput genetic platform aimed at candidate gene mutation screening. Bioinformatics. 23, 2504-2506.

LightScanner and Computer Specifications

Instrument Specifications
Size (w x d x h) 7.8” (20 cm) x 16.5” (42 cm) x 31.8” (81 cm)
Weight 25 kg / 55 lbs
Voltage Input 100-240 VAC, +/-10%, 50-60 Hz with automatic detection and configuration, 2.5 A
Wattage 800 W maximum including computer and monitor.
Cooling time 95° to 60 °C (non-linear)
~ 180 seconds at ambient temperature 25 °C
Heating rate 0.1 °C per second
Single run time 15 minutes, including cool down
Certifications CE, UL (for Electrical Safety)

Sample Handling and Filter System
Sample format Standard 96- or 384-well trays
Reaction volume 5-100 µl
Sample temperature range 40° to 100 °C
Excitation filter 470 +/- 20 nm
Emission filters 510 and 600 nm

Computer and Software
LightScanner Software Accurately performs mutation scanning and genotyping by Hi-Res Melting curve analysis
Computer Pentium-based PC with Windows XP Professional, 17”- 1280 x 1024 graphics monitor and software.
Networking Ethernet connection allows export of data in standard spreadsheet format compatible with LIMS software.

Ordering Information
LightScanner System
Includes instrument, computer, software and Start-up Kit
96-Well Instrument
384-Well Instrument
LSCN-ASY-0011
LSCN-ASY-0001
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