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Genotyping
LightScanner® Assays for Hi-Res Melting™

The following assays have been designed and optimized for use with the LightScanner® System. If you are interested in obtaining a protocol for any of the assays listed below please select the assay(s) and fill in and submit the form. We will send you the information related to that assay.

Many of the SNP genotyping assays use an unlabeled probe, also known as a LunaProbe™, in combination with LCGreen® Plus Dye, to genotype samples.

Send us your assay! If you are interested in a specific gene or SNP, please let us know. We are constantly updating our list and would love to include your gene of choice. Click here to submit your gene.
Step 1
 Click on the type of assay below to go to the specific list of scanning assays:
 

CANCER

CARDIAC

DRUG METABOLIZING ENZYMES - TRANSPORTERS

FLAVIN CONTAINING MONOOXYGENASES

GLUTATHIONE-S-TRANSFERASES

HEMOSTASIS

METABOLISM

NEURONAL

SOLUTE CARRIER FAMILY
Step 2
After you select the desired assays, submit the form on the bottom of this page.

Select Assay
 Assay Gene Assay Type Mutation
METABOLISM
CETP CHOLESTERYL ESTER TRANSFER PROTEIN, PLASMA Whole gene analysis N/A
LIPC LIPASE, HEPATIC Whole gene analysis N/A
LPL LIPOPROTEIN LIPASE Whole gene analysis N/A
LCAT LECITHIN:CHOLESTEROL ACYLTRANSFERASE Whole gene analysis N/A
SCARB1 SCAVENGER RECEPTOR CLASS B, MEMBER 1 Whole gene analysis N/A
ApoF APOLIPOPROTEIN F Whole gene analysis N/A
MCAD ACYL-CoA DEHYDROGENASE, MEDIUM-CHAIN LunaProbe Genotyping Assay 985 A>G
MCAD ACYL-CoA DEHYDROGENASE, MEDIUM-CHAIN LunaProbe Genotyping Assay 199 T>C
MCAD ACYL-CoA DEHYDROGENASE, MEDIUM-CHAIN Whole gene analysis N/A
OTC ORNITHINE CARBAMOYLTRANSFERASE Whole gene analysis N/A
NAGS N-ACETYLGLUTAMATE SYNTHASE Whole gene analysis N/A
GALT GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE Whole gene analysis N/A
MTHFR 5,10-METHYLENETETRAHYDROFOLATE REDUCTASE LunaProbe Genotyping Assay A222V (677C>T)
MTHFR 5,10-METHYLENETETRAHYDROFOLATE REDUCTASE LunaProbe Genotyping Assay E429A (1298A>C)
HFE HEMOCHROMATOSIS LunaProbe Genotyping Assay H63D (187C>G)
HFE HEMOCHROMATOSIS LunaProbe Genotyping Assay C282Y (845G>A)

Select Assay
 Assay Gene Assay Type Mutation
HEMOSTASIS
A1AT ALPHA-1-ANTITRYPSIN PROTEASE INHIBITOR Whole gene analysis N/A
Factor V COAGULATION FACTOR V LunaProbe Genotyping Assay R506Q (1691G>A)
Prothrombin COAGULATION FACTOR II LunaProbe Genotyping Assay 20210 G>A
CFHx9 COMPLEMENT FACTOR H Scanning T/C Y402H, T1277C poly

Select Assay
 Assay Gene Assay Type Mutation
NEURONAL
SOD1x1 SUPEROXIDE DISMUTASE 1 Inquire N/A
SMNx7 SURVIVAL OF MOTOR NEURON 1 (mouse) Inquire N/A
SOX1x1 SRY-BOX 1 (3 fragments) Scanning N/A
SPG4x9 SPASTIN (microtubule disassembly) Scanning N/A
HTR2A SEROTONIN RECEPTOR Scanning x1 102 T>C
BMPR2 BONE MORPHOGENETIC PROTEIN RECEPTOR, TYPE II Whole gene analysis N/A

Select Assay
 Assay Gene Assay Type Mutation
CARDIAC
MYH7x3 MYOSIN, HEAVY CHAIN 7, CARDIAC MUSCLE, BETA Scanning N/A
MYH7x12 MYOSIN, HEAVY CHAIN 7, CARDIAC MUSCLE, BETA Scanning N/A
MYBPC3x3 MYOSIN-BINDING PROTEIN C, CARDIAC Scanning N/A
MYBPC3x4 MYOSIN-BINDING PROTEIN C, CARDIAC Scanning N/A
MYBPC3x6 MYOSIN-BINDING PROTEIN C, CARDIAC Scanning N/A
TNNT2x3,4 TROPONIN T2, CARDIAC (thin filaments, single fragment) Scanning N/A
TNNI3x7 TROPONIN I, CARDIAC (thin filaments) Scanning N/A
TNNI3x8 TROPONIN I, CARDIAC (thin filaments) Scanning N/A

Select Assay
 Assay Gene Assay Type Mutation
DRUG METABOLIZING ENZYMES/TRANSPORTERS
CYP1B1x2 Cytochrome P450, family 1, subfamily B, polypeptide 1 Scanning N/A
CYP1B1x3 Cytochrome P450, family 1, subfamily B, polypeptide 1 Scanning N/A
CYP2D6*6B Cytochrome P450, family 2, subfamily D, polypeptide 6 LunaProbe Genotyping Assay 1707 T>DEL
CYP2D6*4A Cytochrome P450, family 2, subfamily D, polypeptide 6 LunaProbe Genotyping Assay 1846 G>A
CYP2D6*2xN/
*35xN 		Cytochrome P450, family 2, subfamily D, polypeptide 6 LunaProbe Genotyping Assay 2850 C>T
CYP2D6*3 Cytochrome P450, family 2, subfamily D, polypeptide 6 LunaProbe Genotyping Assay 2549 A>del
CYP2C19*2 Cytochrome P450, family 2, subfamily C, polypeptide 19 LunaProbe Genotyping Assay +19154 G>A
CYP2C19*3 Cytochrome P450, family 2, subfamily C, polypeptide 19 LunaProbe Genotyping Assay +17948 G>A
CFTRx10 CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR (ABCC7) Scanning F508del, F508C, I507del, I506V
CFTRx10 CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR (ABCC7) LunaProbe Genotyping Assay F508del, F508C, I507del, I506V
CFTRx11 CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR (ABCC7) LunaProbe Genotyping Assay G542X
ABCB1 ATP-binding cassette, sub-family B (MDR/TAP), member 1 LunaProbe Genotyping Assay -129 T>C
ABCC2 ATP-binding cassette, sub-family C (CFTR/MRP), member 2 LunaProbe Genotyping Assay V417I
(1249 G>T)
ABCC2 ATP-binding cassette, sub-family C (CFTR/MRP), member 2 LunaProbe Genotyping Assay C1515Y
(4544 G>A)
ABCC2 ATP-binding cassette, sub-family C (CFTR/MRP), member 2 LunaProbe Genotyping Assay V1188E
ABCC3 ATP-binding cassette, sub-family C (CFTR/MRP), member 3 LunaProbe Genotyping Assay R1297H
ABCG2 ATP-binding cassette, sub-family G (WHITE), member 2 LunaProbe Genotyping Assay Q141K


Select Assay
 Assay Gene Assay Type Mutation
SOLUTE CARRIER FAMILY
SLC22A1 Solute carrier family 22 (organic cation transporter), member 1 LunaProbe Genotyping Assay G465R
(32870 G > A)
SLC22A1 Solute carrier family 22 (organic cation transporter), member 1 LunaProbe Genotyping Assay R61C
(286 C>T)
SLC22A1 Solute carrier family 22 (organic cation transporter), member 1 LunaProbe Genotyping Assay L160F
(585 G>C)
SLC22A2 Solute carrier family 22 (organic cation transporter), member 2 LunaProbe Genotyping Assay S270A
(978 T>G)
SLCO1B1 Solute carrier organic anion transporter family, member 1B1 LunaProbe Genotyping Assay N130D
(484 A>G)
SLCO1B1 Solute carrier organic anion transporter family, member 1B1 LunaProbe Genotyping Assay L643F
(2063 A>C)
SLCO1B1 Solute carrier organic anion transporter family, member 1B1 LunaProbe Genotyping Assay V174A
(617 T>C)
SLCO2B1 Solute carrier organic anion transporter family, member 2B1 LunaProbe Genotyping Assay S486F
(1721 C>T)
SLCO2B1 Solute carrier organic anion transporter family, member 2B1 LunaProbe Genotyping Assay V210M
(865 G>A)
SLCO2B1 Solute carrier organic anion transporter family, member 2B1 LunaProbe Genotyping Assay Q312R
(1199 A>G)
SLC22A1 Solute carrier family 22 (organic cation transporter), member 1 LunaProbe Genotyping Assay G465R
(32870 G > A)
SLC22A5 Solute carrier family 22, member 5 Whole gene analysis N/A

Select Assay
 Assay Gene Assay Type Mutation
FLAVIN CONTAINING MONOOXYGENASES
FMO3 Flavin containing monooxygenase 3 LunaProbe Genotyping Assay E308G
(21443 A>G)
FMO3 Flavin containing monooxygenase 3 LunaProbe Genotyping Assay E158K (15167G>A)
FMO3 Flavin containing monooxygenase 3 LunaProbe Genotyping Assay V257M
(18281 G>A)

Select Assay
 Assay Gene Assay Type Mutation
GLUTATHIONE-S-TRANSFERASES
GSTA2 Glutathione S-transferase A2 LunaProbe
Genotyping Assay 		S112T
(448 G>C)
GSTA2 Glutathione S-transferase A2 LunaProbe
Genotyping Assay 		P110S
(441 C>T)
GSTA2 Glutathione S-transferase A2 LunaProbe Genotyping Assay E210A
(742 A>C)
GSTM3 Glutathione S-transferase M3 (brain) LunaProbe
Genotyping Assay		 V224I
(801 G>A)
GSTP Glutathione S-transferase pi LunaProbe
Genotyping Assay 		A114V
(370 C>T)

Select Assay
 Assay Gene Assay Type Mutation
CANCER
AXLx5 AXL RECEPTOR TYROSINE KINASE Scanning N/A
BRIP1 BRCA1-INTERACTING PROTEIN 1 Scanning N/A
BRAFx11 V-RAF MURINE SARCOMA VIRAL ONCOGENE HOMOLOG B1 Scanning N/A
BRAFx15 V-RAF MURINE SARCOMA VIRAL ONCOGENE HOMOLOG B1 Scanning N/A
EGFRx18 EPIDERMAL GROWTH FACTOR RECEPTOR Scanning N/A
EGFRx19 EPIDERMAL GROWTH FACTOR RECEPTOR Scanning N/A
EGFRx20 EPIDERMAL GROWTH FACTOR RECEPTOR Scanning N/A
EGFRx20 EPIDERMAL GROWTH FACTOR RECEPTOR LunaProbe Genotyping Assay 2361 G>A
EGFRx21 EPIDERMAL GROWTH FACTOR RECEPTOR Scanning N/A
ERBB2x23 V-ERB-B2 AVIAN ERYTHROBLASTIC LEUKEMIA VIRAL ONCOGENE HOMOLOG 2 Scanning N/A
ERBB2x24 V-ERB-B2 AVIAN ERYTHROBLASTIC LEUKEMIA VIRAL ONCOGENE HOMOLOG 2 Scanning N/A
FGF3Rx6 FIBROBLAST GROWTH FACTOR RECEPTOR 3 Scanning N/A
FGF3Rx8 FIBROBLAST GROWTH FACTOR RECEPTOR 3 Scanning N/A
FGF3Rx13 FIBROBLAST GROWTH FACTOR RECEPTOR 3 Scanning N/A
FGF3Rx17 FIBROBLAST GROWTH FACTOR RECEPTOR 3 Scanning N/A
Irf4 INTERFERON REGULATORY FACTOR 1 Scanning N/A
JAK1x13 JANUS KINASE 1 Scanning N/A
JAK2x14 JANUS KINASE 2 Scanning N/A
JAK2x22 JANUS KINASE 2 Scanning N/A
KITx2 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
KITx8 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
KITx9 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
KITx11 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
KITx12/13 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
KITx15 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
KITx17 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
MSH2 MutL, E. COLI, HOMOLOG OF, 2 Whole gene analysis N/A
MLH1 MutL, E. COLI, HOMOLOG OF, 1 Whole gene analysis N/A
METx14 MET PROTOONCOGENE Scanning N/A
METx16 MET PROTOONCOGENE Scanning N/A
METx17 MET PROTOONCOGENE Scanning N/A
METx18 MET PROTOONCOGENE Scanning N/A
METx19 MET PROTOONCOGENE Scanning N/A
PDGFRAx10 PLATELET-DERIVED GROWTH FACTOR RECEPTOR, ALPHA Scanning N/A
PDGFRAx12 PLATELET-DERIVED GROWTH FACTOR RECEPTOR, ALPHA Scanning N/A
PDGFRAx18 PLATELET-DERIVED GROWTH FACTOR RECEPTOR, ALPHA Scanning N/A
PDPK1x3 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x4 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x5 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x6 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x7 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x8 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x9 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x10 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x11 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x12 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x13 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x14 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PIK3Cax2 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax3 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax5 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax8 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax10 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax14 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax19 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax21 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
ROS1 V-ROS AVIAN UR2 SARCOMA VIRUS ONCOGENE HOMOLOG 1 Scanning N/A
RETx10 REARRANGED DURING TRANSFECTION PROTOONCOGENE Scanning N/A
RETx16 REARRANGED DURING TRANSFECTION PROTOONCOGENE Scanning N/A
K-Rasx2 V-KI-RAS KIRSTEN RAT SARCOMA VIRAL ONCOGENE Scanning N/A
K-Rasx3 V-KI-RAS KIRSTEN RAT SARCOMA VIRAL ONCOGENE Scanning N/A
TP53x5 TUMOR PROTEIN p53 Scanning N/A

Please submit the following form to receive the protocols for the selected scanning assays.
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If you encounter any problems with this process, please contact Idaho Technology's technical support representative at 1-800-735-6544 or click here.
These assays are provided for informational purposes only and may be covered by intellectual property of third parties. No license is expressed or implied by the provision of assay information. The assays listed here are not validated for human clinical / diagnostic use. The genes and technologies that are involved with the assays posted may be covered by 3rd party intellectual property. LightScanner Master Mix is sold for research use only.
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