Idaho Technology Inc. - High Sensitivity Master Mix

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Genotyping

High Sensitivity Master Mix

Designed for Genotyping using Small Amplicons

The LightScanner® High Sensitivity Genotyping Master Mix is a specialized master mix containing LCGreen Plus® dye and internal temperature calibrators. Many genotyping studies include samples where the polymorphism consists of a base pair neutral change (A>T, G>C). Inclusion of these temperature calibrators in this master mix enables differentiation of even these subtle differences.

Benefits High Sensitivity Master Mix

The panels below illustrate the genotyping results obtained with (Panel a) and without (Panel b) the LightScanner High Sensitivity Genotyping Master Mix for a G>C SNP in a 42 bp product. These results demonstrate the benefits of using the specialized master mix.

Melting Peak Comparison

High Sensitivity Master Mix Specifications

Kit Details
Content	1x100 Rxns of 2.5 x Master Mix
	1.5 mL of 10mM MgCl2
	1.5 mL of Reagent Grade Water
	1 LightScanner Master Mix User’s Guide
Shipping and Storage	Store at -20 ºC for up to 1 year
	Once thawed, store at 4 ºC for 2 weeks
	LightScanner Master Mix is shipped on dry ice

Ordering Information
	Catalog Number
High Sensitivity Genotyping Master Mix (100 Reactions)	HRLS-ASY-0008
High Sensitivity Genotyping Master Mix (500 Reactions)	HRLS-ASY-0009

References
Gundry CN, Dobrowolski SF, Martin YR, Robbins TC, Nay LM, Boyd N, Coyne T, Wall MD, Wittwer CT, Teng DH. Base-pair neutral homozygotes can be discriminated by calibrated high-resolution melting of small amplicons. Nucleic Acids Res. 2008 Apr 29. [Epub ahead of print]PMID: 18448472 [PubMed - as supplied by publisher]

Liew, M., et al. (2004) Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons. Clin Chem. Jul;50(7):1156-64.

Liew, M., et al. (2007) Closed-tube SNP genotyping without labeled probes/a comparison between unlabeled probe and amplicon melting. Am. J. Clin. Pathol. 127, 341-348

Seipp, M. T. et al. (2007) Unlabeled Oligonucleotides as Internal Temperature Controls for Genotyping by Amplicon Melting. J Mol Diagn., 9, 284-9

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New Product

The High Sensitivity Master Mix is a new product. It is compatible with both the LightScanner® and HR-1® instruments.