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LightScanner® System
The LightScanner is the first and only instrument to utilize Hi-Res Melting® for mutation scanning and genotyping in a 96- or 384-well plate system. This exciting technology, licensed exclusively to Idaho Technology, is based on U.S. Patent No. 7,387,887 (Nucleic Acid Melting Analysis with Saturation Dyes) and U.S. Patent Publication 2006-0019253 (Amplicon Melting Analysis with Saturation dyes).
The LightScanner is unsurpassed in its temperature control and data acquisition rates. With superb sensitivity and specificity, this instrument is designed to meet the needs of high throughput scanning projects. Simply add LCGreen® dye to the initial PCR reaction– then transfer the PCR plate to the LightScanner for melting. Intuitive analysis software groups the results and provides automatic results. |
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Benefits from a Complete Product Solution
| Instrument |
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Chemistry |
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Results in less than five minutes |
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Optimized Master Mix for superior PCR performance for Hi-Res Melting applications |
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Hi-Res Melting is non-destructive allowing samples to be recovered for further analysis |
| Support |
Software |
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Obtain quick assistance through phone and online help desks |
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Software automatically analyses each sample and defines the standards and variants |
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Work directly with highly experienced scientists |
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Simple data management tools |
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Primer design software |
LightScanner Software
Our comprehensive software package provides both assay design and data analysis modules. The primer design module streamlines assay design for Hi-Res Melting. The analysis software features Call-IT®, a patented scientific method where the software critically analyses each sample, defines and groups wild-types and variants. This software also provides users with simple data management tools.
LightScanner and Computer Specifications
| Instrument Specifications |
| Size (w x d x h) |
7.8” (20 cm) x 16.5” (42 cm) x 31.8” (81 cm) |
| Weight |
25 kg / 55 lbs |
| Voltage Input |
100-240 VAC, +/-10%, 50-60 Hz with automatic detection and configuration, 2.5 A |
| Wattage |
800 W maximum including computer and monitor. |
| Cooling time |
95° to 60 °C (non-linear)
~ 180 seconds at ambient temperature 25 °C |
| Heating rate |
0.1 °C per second |
| Single run time |
15 minutes, including cool down |
| Sample Handling and Filter System |
| Sample format |
Standard 96- or 384-well trays |
| Reaction volume |
5-100 µl |
| Sample temperature range |
40° to 100 °C |
| Excitation filter |
470 +/- 20 nm |
| Emission filters |
510 and 600 nm |
| Computer and Software |
| LightScanner Software |
Accurately performs mutation scanning and genotyping by Hi-Res Melting curve analysis |
| Computer |
Pentium-based PC with Windows XP Professional, 17”- 1280 x 1024 graphics monitor and software. |
| Networking |
Ethernet connection allows export of data in standard spreadsheet format compatible with LIMS software.
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| Ordering Information |
LightScanner System
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96-Well Instrument |
384-Well Instrument |
LSCN-ASY-0011 |
LSCN-ASY-0001 |
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LightScanner Automation |
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Watch as Lab Services new automated robot loads and unloads plates from a LightScanner System.
Click here to see the demo |
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