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LCGreen® Melting Dyes
LCGreen dyes are specifically designed for high-resolution melting curve analysis to detect DNA sequence variants. The addition of LCGreen dyes increase the melting temperature of DNA by 1-3 °C and may requre adjustment of cycling parameters. The dyes are manufactured exclusively by Idaho Technology and their chemical structures are unique among the scientific and patent literature.
LCGreen dyes are tailored specifically for Hi-Res Melting and have the unique ability to detect heteroduplexes during melting analysis after PCR. Just add the dye to your sample before PCR. LCGreen dyes are extremely stable, do not inhibit PCR, and are "saturation" dyes that can detect multiple PCR products in a mixture during melting analysis. |
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LCGreen Plus+
 LCGreen PLUS is a new member of the dye family tailored for use in melting instruments with 96- or 384-well microtiter plates. It has superb fluorescence intensity and can be used with other fluorescence based PCR detection systems such as the Roche LightCycler®. For optimal performance, the use of a high-resolution melting instrument is required.
LCGreen I
LCGreen I is a dsDNA binding dye used for Hi-Res Melting curve analysis using Idaho Technology’s HR-1™ instrument. This innovative dye is manufactured exclusively by Idaho Technology and is designed specifically for Hi-Res Melting analysis to detect DNA sequence variants (SNPs, Insertions / Deletions).
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Kit Details
| LCGreen Plus+ |
| Number of Reactions |
10X Solution |
| 1000 Reactions |
1 mL |
| 10,000 Reactions |
10 x 1 mL |
| LCGreen I |
| Description |
Reaction Buffer 10X |
dNTPs 10X |
BSA 10X |
Enzyme diluent 1X |
100 Reactions
100 µl |
1 ml - 10, 20, or 30 mM
MgCI 2 |
1 ml |
1 ml |
1 ml |
400 Reactions
4 x 100 µl |
1 ml - 10, 20, or 30 mM
MgCI 2 |
1 ml |
1 ml |
1 ml |
1,000 Reactions
4 x 250 µl |
1 ml - 10, 20, or 30 mM
MgCI 2 |
1 ml |
1 ml |
1 ml |
10,000 Reactions
40 x 250 µl |
1 ml - 10, 20, or 30 mM
MgCI 2 |
1 ml |
1 ml |
1 ml |
| Ordering Information |
| LCGreen Plus Melting Dye |
No. of Rxns |
1,000 |
10,000 |
Larger sizes
Catalog No. Inquire |
Catalog No. |
BCHM-ASY-
0005 |
BCHM-ASY-0006 |
| LCGreen I Melting Dye |
No. of Rxns |
100 |
400 |
1,000 |
10,000 |
Catalog No. |
Included with
instrument purchase |
BCHM-ASY-0003 |
BCHM-ASY-0001 |
BCHM-ASY-0004 |
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