BioDefense
Food Security Testing
Water Security Testing
Mutation Discovery
Genotyping
  - LightScanner
  - HR-1
  - RapidCycler 2
  - LightScanner Master Mixes
  - LCGreen Melting Dyes
  - LightScanner Assays
  - Custom Probes & Primers
  - Reagents, Buffers & Capillary Tubes
Genotyping

Genotyping is possible with Hi-Res Melting eliminating the need for labeled probes. It is achieved by Hi-Res Melting™ in two ways:

  1. With unlabeled 3' blocked probes data from the probe and PCR product can be obtained simultaneously.
  2. Small amplicons often as short as 50 ~ 60 bp with primers just flanking the SNP. As this application relies on subtle differences in melting temperature, it is best performed on our instruments which have the best temperature precision on the market for melting.

Use the links below to learn more about our complete line of Genotyping products.

 
Instrument

LightScanner Instrument
LightScanner® System

  • Hi-Res Melting Instrument
  • 96- or 384-well plate system
  • Results in less than five minutes
  • Software automatically analyses each sample and defines the standards and variants

HR-1 Instrument
HR-1® System

  • Hi-Res Melting Instrument
  • Single sample capillary melter
  • Up to 45 samples per hour with a 0.3°C ramp rate
  • Easy-to-use, low cost mutation discovery instrument

RapidCycler 2 Instrument
RapidCycler® 2 Instrument

  • High-Speed PCR
  • 30 cycles in under 15 minutes
  • 5-20 µl using 1.5 mm capillary tubes
  • 48 samples per run, over 1,500 samples per 8 hour day
Chemistry

LightScanner Master Mix
LightScanner Master Mix

  • A pre-optimized solution
    specifically designed to deliver superior PCR performance for Hi-Res Melting applications on the LightScanner Instrument

High Sensitivity Master Mix
High Sensitivity Master Mix

  • The LightScanner® High Sensitivity Genotyping Master Mix is a specialized master mix containing LCGreen Plus® dye and internal temperature calibrators.

Warfarin Genotyping Reagents
Warfarin Genotyping Reagents

  • Our Warfarin Genotyping Reagents (ASR) provide a fast and effective solution for genotyping.

LCGreen + Melting Dye
LCGreen® Melting Dyes

  • LCGreen dyes are specifically designed for high-resolution melting curve analysis to detect DNA sequence variants (mutations, polymorphisms, etc).

LCGreen + Melting Dye
Reagents, Buffers and Capillary Tubes

 

 
Services

LightScanner Assay Protocols
LightScanner Assays

  • Free assay design protocols that have been optimized for Hi-Res Melting.

IT BioChem - Custom Probes, Primers and Melting Dyes
Custom Probes & Primers

  • Order custom designed probes, primers and melting dyes for DNA amplification, mutation and detection.
  • ASR grade oligos
  • Manufactured under cGMP