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| How it Works
 The Idaho Technology FilmArray is the latest in user-friendly automated
multiplex PCR. The FilmArray integrates sample preparation, amplification, detection, and analysis into one easy to use system
capable of massively multiplexed PCR. Fully automated, multiplex
PCR capable of detecting greater than 100 targets in one sample in less than one hour.
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Automated Protocol
Minimize hands on time – start the run and walk away
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Integrated Sample Preparation
Bypass upstream DNA/RNA extraction instruments
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Automated analysis of results
Eliminate subjective analysis
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Speed
Reduce turn around time – results in less than an hour
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| The FilmArray Pouch
All the necessary reagents for sample preparation, reverse transcription-PCR, PCR, and detection are stored in the FilmArray pouch in a freeze dried format. Prior to a run, the user injects water and unprocessed sample into the pouch. The FilmArray instrument does the rest.
After extracting and purifying nucleic acids from the unprocessed sample the FilmArray performs a nested multiplex PCR that is executed in two stages. During the first stage, or PCR 1, the FilmArray performs a single, large volume, massively multiplexed reaction. The products from PCR 1 are then diluted and combined with a fresh, primer-free master mix. Aliquots of this second master mix solution are then distributed to each well of the array. Each well of the array is pre-spotted with a single set of primers. The second stage PCR, or PCR 2, is performed in singleplex fashion in each well of the array.
Using amplification and melting curve data, the FilmArray software automatically generates a result for each target.
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The FilmArray System
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Inject unprocessed sample and water, start the run and walk away
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System integrates sample preparation, amplification, detection and analysis
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All necessary reagents are freeze dried in one pouch and are stable at room temperature
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Closed system prevents cross-contamination
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 Each run contains internal process controls for RNA extraction, DNA extraction, RT-PCR, PCR 1, dilution, and PCR 2
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Advanced software runs the system and automatically analyzes and reports results at the completion of a run
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Multiplexed testing analyzes up to 120 tests per sample
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Rapid results in 1 hour from sample injection
- Simple syringe loading, no pipetting or centrifugation required
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FilmArray Instrument Specifications
| Specifications |
| Sample Handling |
- Sample Types: Nasopharyngeal Swab, Nasopharyngeal Aspirate
- Sample Volume: Up to 100 µL
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| Analysis Specifications |
- Sensitivity: Comparable to common single -plex molecular methods
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| Instrument Specifications |
- Power Requirements: 90-264 VAC, 10 A
- Size: 25.4 x 39.3 x 16.5 cm (10 x 15.5 x 6.5 in.)
- Weight: 9 kg (20 lb.)
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| Performance Parameters |
- Instrument/System set-up time ≤ 5 min.
- Sample run time ≤ 1 hour
- Analyzes up to 120 tests per sample
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| Environmental Specification |
- Operating: 15 °C to 30 °C at 20 to 80% humidity
- Storage: -30 °C to 65 °C
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| Desktop Software |
- Windows-based instrument control and data analysis software
- Barcode reader for data input
- Automated qualitative analysis and reporting
- Separate advanced analysis software
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